| Ph.D. Students
Stawiarz Magdalena M.Sc.
ORCID
Supervisor: Artur Kowalik Ph.D., Assoc. Prof.
Research topic: The subject of my research concerns the application of the Next Generation Sequencing (NGS) technique in research on the Minimal Residual Disease (MRD) in the course of acute myeloid leukemia. MRD is characterized by the presence of a small number of neoplastic cells (during remission or treatment) leading to frequent relapses. This condition is difficult to diagnose using standard methods, while the use of NGS allows for the detection of changes with high sensitivity. The aim of my research is to fully understand the meaning of genetic diversity (mutations and variability of the expression level) associated with the discussed disease entity. This may contribute to the development of modern and personalized therapeutic methods as well facilitate diagnosis and monitoring of MRD.
Methods: NGS, PCR, RT-qPCR
Lach Karolina M.Sc.
ORCID
Supervisor: Ciepluch Karol Ph.D., Assoc. Prof.
Auxiliary Supervisor: Łysek-Gładysińska Małgorzata Ph.D.
Research topic: The aim of this dissertation is to characterize metallodendrimers and their enhancing antibacterial properties of phage endolysin against Gram-negative bacteria. This characterization aims to study the effect of metallodendrimers as well as their complex with endolysin and select the most stable complex showing the best effect in terms of the intensity of permeabilization of the outer bacterial membrane on the liposome model, degradation processes of peptidoglycan and inhibition of bacterial biofilm formation. In the next part of the study, the cytotoxic properties of selected metallodendrimers and complex with endolysin will be investigated on in vitro culture of eukaryotic cells.
Methods: in vitro studies, spectrofluorimetry, fluorescence microscopy, TEM, SEM, flow cytometry
Kacper Kalwat M.Sc
ORCID
Supervisor: prof. Michał Arabski (biology), prof. Javier De La Mata (chemistry)
Research topic: Melanoma is one of the fastest-growing skin cancers worldwide. According to WHO data, more than 320,000 new cases are diagnosed annually, and approximately 57,000 deaths are recorded. In countries with high exposure to UV radiation the incidence has increased several times over the past decades. Increased sunlight due to climate change may also be a contributing factor. The aim of the project is therefore the synthesis and evaluation of the cytotoxicity of a polymer-based dressing containing a conjugate of a carboxydendrimer modified with the specific peptide. The peptide binds to receptors, which are overexpressed on melanoma cells. The proposed dressing will be designed as a targeted system for cancer cells.
Methods: in vitro cultivation methods, RT-PCR, Western blot, flow cytometry, cytotxicity tests, chemical synthesis and modifications |
